Fluorescent Ceramides

The fluorescent functional group nitrobenzoxadiazole (NBD), with an absorption of about 467nm and an emission of about 439nm, is a well known and established label for in vivo and in vitro research. NBD has been shown to have only a small influence on lipid adsorption into cells and cellular membranes making it useful for cellular studies.1,2

References:

  1. D. Moffat and J. Kusel “Fluorescent lipid uptake and transport in adult Schistosoma mansoni” Parasitology, Vol. 105(1) pp. 81-89, 1992
  2. P. Sengupta et al. “Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles” Biochimica et Biophysica Acta, Vol. 1778(1) pp. 20-32, 2008