Fluorescent Standards

Matreya has developed fluorescent standards that can be detected in cultures and in biological systems, making them ideal for studies involving the metabolism of sphingolipids. These lipid analogs are very useful for determining the localization of various sphingolipids in membranes and organelles.⁽¹⁾ The NBD fluorescent group attached to hexanoic acid has been shown to be readily taken up by cells and used in the biosynthesis of more complex sphingolipids.⁽²⁾

References:

1. A. Merrill Jr. Sphingolipid and Glycosphingolipid Metabolic Pathways in the Era of Sphingolipidomics" Chemical Reviews, Vol. 111 pp. 6387-6422, 2011
2. N. Lipsky and R. Pagano, "Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: endogenously synthesized sphingomyelin and glucocerebroside analogues pass through the Golgi apparatus en route to the plasma membrane" Journal of Cell Biology, Vol. 100 pp. 27-34, 1985