Application Notes:
Digalactosyldiglyceride (DGDG) is one of the main lipids of the membranes of chloroplasts and it is one of the most
abundant lipids in all photosynthetic tissues, including those of algae and some bacteria. In plants, it is much less prevalent in
non-photosynthetic tissues. DGDG is formed by the de-phosphorylation of phosphatidic acid or phosphatidylcholine and the
subsequent reaction with two 5-diphosphate(UDP)-galactoses. Digalactosyldiglycerides form a lipid bilayer in the lipid
membrane whereas monogalactosyldiglycerides (MGDG) do not. A proper ratio of these two diglycerides is critical to
membrane function. DGDG is important in photosynthesis although its role is not fully understood and is undergoing much
active research. In cells undergoing photosynthesis, the photosystem I complex contains three moles of MGDG and one mole
of phosphatidylglycerol while photosystem II contains four moles of DGDG. The proper ratio of lipids is needed for the
effective crystallization of the light-harvesting complex II. However, an inability for plants to produce DGDG does not result
in lethal phenotypes although a lack of phosphatidylglycerol does. It has been suggested that DGDG has a role in the
activation of ATPase and it has an elevated expression in the peribacteroid membrane of nitrogen-fixing nodules of legumes.1
DGDG functions in the formation of thylakoid membrane stacks, chloroplast membrane biogenesis and protein import into
chloroplasts.2 A recent report has suggested that DGDG may also have anti-cancer properties when administered orally .3
This highly pure product from Matreya is ideal for the research of digalactosyldiglyceride functions and identification.
References:
1. Nicole Gaude, “The Galactolipid Digalactosyldiacylglycerol Accumulates in the Peribacteroid Membrane of Nitrogen-fixing Nodules of Soybean and
Lotus” Journal of Biological Chemistry, Vol. 279:33 pp. 34624-34630, 2004
2. John E. Froehlich, Christoph Benning, and Peter Do¨rmann “The Digalactosyldiacylglycerol (DGDG) Synthase DGD1 Is Inserted into the Outer Envelope
Membrane of Chloroplasts in a Manner Independent of the General Import Pathway and Does Not Depend on Direct Interaction with
Monogalactosyldiacylglycerol Synthase for DGDG Biosynthesis” Journal of Biological Chemistry, Vol. 276:34 pp. 31806-31812, 2001
3. Naoki Maeda “Anti-Tumor Effect of Orally Administered Spinach Glycolipid Fraction on Implanted Cancer Cells, Colon-26, in Mice” Lipids, Vol. 43 pp.
741-748, 2008