Application Notes:
This fatty acid methyl ester mixture contains branched and straight chain fatty acids in chloroform for qualitative
identification. Knowledge of the fatty acid content of bacteria can be of great benefit in understanding microbials and can be
of great nutritional importance in animals.1 Understanding the role of enzymes and regulatory pathways in human pathogens
is important in drug development.2 Microbial fatty acid profiles are unique from one species to another and can therefore be
used in the determination of bacterial identity.3 This is a qualitative mixture and should not be used for quantitative
determinations.
All materials are analyzed to verify their identity and to determine their purity. All analytes are 98+% pure. This standard is
accurately prepared by gravimetric technique (+/- 0.5%) and all glassware is class A rated. Ampules are purged with
nitrogen/argon before and after filling and chilled before being flame sealed. Store ampules sealed as received and process
without delay immediately after opening the ampule.
References:
1. M. Or-Rashid, N. Odongo and B. McBride, “Fatty acid composition of ruminal bacteria and protozoa, with emphasis on conjugated linoleic acid, vaccenic
acid, and odd-chain and branched-chain fatty acids” Journal of Animal Science vol. 85 pp. 1228, 2007
2. Y-M Zhang, S. White, and C. Rock “Inhibiting Bacterial Fatty Acid Synthesis” The Journal of Biological Chemistry vol. 281 pp. 17541, 2006
3. N. Rozès, S. Garbay, M. Denayrolles, A. Lonvaud-Funel “A rapid method for the determination of bacterial fatty acid composition” Applied Microbiology
vol. 3(17) pp. 126, 1993