Application Notes:
This fatty acid reference mixture contains saturated, mono and poly-unsaturated fatty acid methyl esters for the qualitative
identification and quantitation of samples. This AOCS reference mixture is especially ideal as a standard for lard, beef
tallow, mutton tallow, and palm oils. By studying problems with the quantitative analysis of animal and vegetable oils and
fats, the American Oil Chemists’ Society has found certain mixtures to be useful as reference standards. The composition of
each mixture is similar to the fatty acid distribution of certain oils. This is an excellent standard for identifying unknown fatty
acid isomers in samples.
All materials are analyzed to verify their identity and to determine their purity. All analytes are 99% pure. This standard is
accurately prepared by gravimetric technique (+/- 0.5%) and all glassware is class A rated. Ampules are purged with
nitrogen/argon before and after filling and chilled before being flame sealed. Store ampules sealed as received and process
without delay immediately after opening the ampule.
References:
1. Z. Li, T. Gu, B. Kelder and J. J. Kopchick “Analysis of fatty acids in mouse cells using reversed-phase high-performance liquid chromatography”
Chromatographia, Oct. Vol. 54 pp. 463-467 2001
2. L. D. Metcalfe, A. A. Schmitz, J. R. Pelka “The Rapid Preparation of Fatty Acid Esters from Lipids for Gas Chromatographic Analysis” Analytical
Chemistry, March, Vol. 38(3) pp. 514-515 1966