Application Notes:
As this product is derived from a natural source, there may be variations in the sphingoid backbone.
Gangliosides1 are acidic glycosphingolipids that form lipid rafts in the outer leaflet of the cell plasma membrane, especially
in neuronal cells in the central nervous system.2 They participate in cellular proliferation, differentiation, adhesion, signal
transduction, cell-to-cell interactions, tumorigenesis, and metastasis.3 The accumulation of gangliosides has been linked to
several diseases including Tay-Sachs and Sandhoff disease while an autoimmune response against gangliosides can lead to
Guillain-Barré syndrome. Miller-Fisher syndrome, a variant of Guillain-Barré syndrome, is an autoimmune disease
characterized by the presence of anti-GQ1b ganglioside antibodies. Studies of these antibodies reveal large disruptions of
Schwann cells. GQ1b has been shown to enhance Ig production of human peripheral blood mononuclear cells and to
selectively enhance Th1 cytokine production while suppressing Th2 production. GQ1b has also been shown to enhance PHAinduced
IL-2 secretion of peripheral blood T cells while it decreases PHA-induced IL-4 and IL-5 secretion. GQ1b suppresses
PHA-induced increases in cAMP levels in T cells and suppresses PHA-stimulated adenylate cyclase activity in T cells.4
References:
1. L. Svennerholm, et al. (eds.), Structure and Function of Gangliosides, New York, Plenum, 1980
2. T. Kolter, R. Proia, K. Sandhoff, Combinatorial Ganglioside Biosynthesis. J. Biol. Chem., July Vol. 277, No. 29, pp. 25859-25862, 2002
3. S. Birkle, G. Zeng, L. Gao, R. K. Yu, and J. Aubry. Role of tumor-associated gangliosides in cancer progression. Biochimie, 85, 455–463, 2003
4. N. Kanda and S. Watanabe “Gangliosides GD1b, GT1b, and GQ1b Enhance IL-2 and IFN-g Production and Suppress IL-4 and IL-5 Production in
Phytohemagglutinin-Stimulated Human T Cells” The Journal of Immunology, Vol. 166 pp. 72-80, 2001